huvec cell complete medium  (Procell Inc)

Journal: Journal of medical virology

Article Title: SARS-CoV-2 pseudovirus infectivity and expression of viral entry-related factors ACE2, TMPRSS2, Kim-1 and NRP-1 in human cells from respiratory, urinary, digestive, reproductive and immune systems

doi: 10.1002/jmv.27244

Figure Lengend Snippet: Cell lines used in the present study

Article Snippet: Table 1 Cell name Origin Source of cell Culture medium Respiratory system JHU‐029 Laryngeal squamous cell carcinoma CVCL_5993 RPMI‐1640 complete medium NCL H460 Large cell lung cancer ATCC HTB‐177 RPMI‐1640 complete medium NCL H322 Large cell lung cancer ATCC CRL­5806 DMEM complete medium NCL H520 Large cell lung cancer ATCC HTB‐182 DMEM complete medium A549 Lung adenocarcinoma ATCC CRM‐CCL‐185 DMEM complete medium Primary human lobar bronchial epithelial cells (HLBEC) Lobar bronchial tissue Lifeline® Cell Technology Lifeline® BronchiaLife™ Medium Primary human small airway epithelial cells (HSAEC) Small airway tissue Lifeline® Cell Technology Lifeline® BronchiaLife™ Medium Urinary system 769‐P Renal cell adenocarcinoma ATCC CRL‐1933 RPMI‐1640 complete medium 768‐O Renal cell adenocarcinoma ATCC CRL‐1932 RPMI‐1640 complete medium A498 Renal cell adenocarcinoma ATCC HTB‐44 RPMI‐1640 complete medium Caki‐1 Renal cell carcinoma ATCC HTB‐46 DMEM complete medium ACHN Renal cell adenocarcinoma ATCC CRL‐1611 DMEM complete medium HRC45 Renal cell carcinoma CVCL_IS24 DMEM complete medium HRC63 Renal cell carcinoma CVCL_IS25 DMEM complete medium HRC59 Renal cell carcinoma FCCC DMEM complete medium Immune system BCP‐1 Primary effusion lymphoma ATCC CRL‐2294 RPMI‐1640 complete medium BC‐3 Primary effusion lymphoma ATCC CRL‐2277 RPMI‐1640 complete medium BJAB Primary effusion lymphoma CVCL_5711 RPMI‐1640 complete medium THP‐1 Acute monocytic leukemia ATCC TIB‐202 RPMI‐1640 complete medium Digestive system Huh‐7 Hepatocellular carcinoma cell JCRB0403 DMEM complete medium PCI‐13 Oral cavity squamous cell carcinoma CVCL_C182 RPMI‐1640 complete medium UD‐SCC‐2 Hypopharyngeal squamous cell carcinoma CVCL_E325 RPMI‐1640 complete medium Reproductive system HUVEC Umbilical Vein Endothelial Cells ATCC PCS‐100‐010 ECBM(Cell applications, 210‐490) T47D Ductal carcinoma ATCC HTB‐133 RPMI‐1640 complete medium MCF‐7 Breast adenocarcinoma ATCC HTB‐22 RPMI‐1640 complete medium Open in a separate window Note : DMEM or RPMI‐1640 complete medium is made of DMEM or RPMI‐1640 basal medium with 10% fetal bovine serum (FBS) and 1% Penicillin‐Streptomycin 100× Solution (25‐512, GenClone).

Techniques: Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: The Nucleolar Protein Nucleophosmin Is Physiologically Secreted by Endothelial Cells in Response to Stress Exerting Proangiogenic Activity Both In Vitro and In Vivo

doi: 10.3390/ijms22073672

Figure Lengend Snippet: ( A–C ) . Western Blotting analysis of cytoplasm and nuclear fraction of nucleophosmin (NPM) in human vein endothelial cells (HUVEC) after serum starvation. ( A ) Representative blot displaying the shuttle of NPM. Lamin B and α-tubulin have been employed as nuclear and cytoplasmic protein, respectively. ( B ) The densitometry analysis shows that NPM translocates from nucleus to the cytoplasm after serum deprivation (w/o Serum) but not in the presence of serum (w/Serum). Data are the results of 3 independent experiments. * p < 0.05, *** p < 0.001. ( C ) Immunofluorescent staining of HUVEC confirming a delocalized NPM in the cytoplasm in the absence of serum (w/o Serum) but not in presence of serum (w/Serum). NPM and DAPI stain green and blue, respectively. Scale bar is displayed.

Article Snippet: Cells were cultured in HUVEC complete growth medium (EGM TM -2 Endothelial Cell Growth Medium-2 BulletKit TM Lonza, Cat. N. CC-3162.

Techniques: Western Blot, Staining

Journal: International Journal of Molecular Sciences

Article Title: The Nucleolar Protein Nucleophosmin Is Physiologically Secreted by Endothelial Cells in Response to Stress Exerting Proangiogenic Activity Both In Vitro and In Vivo

doi: 10.3390/ijms22073672

Figure Lengend Snippet: ( A , B ). Secretion of NPM by HUVEC in serum starvation conditions and in absence of cell necrosis. ( A ) Active release of NPM into the microenvironment of HUVEC according to a time course (6, 18, 24 h) of serum deprivation (w/Serum) and measured by ELISA, showing a gradual increase of the protein in the culture media of endothelial cells at 18 and 24 h compared to 6 h. Data are the results of 4 independent experiments (technical duplicates). * p < 0.05, ** p < 0.01. ( B ) Concentration of the enzyme lactate dehydrogenase (LDH) displaying the absence of cell necrosis in both conditions (with Serum, w/Serum and without Serum, w/o Serum). Data are the results of 3 independent experiments (technical duplicates). O.D. optical density.

Article Snippet: Cells were cultured in HUVEC complete growth medium (EGM TM -2 Endothelial Cell Growth Medium-2 BulletKit TM Lonza, Cat. N. CC-3162.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: The Nucleolar Protein Nucleophosmin Is Physiologically Secreted by Endothelial Cells in Response to Stress Exerting Proangiogenic Activity Both In Vitro and In Vivo

doi: 10.3390/ijms22073672

Figure Lengend Snippet: ( A – C ). Biological effects of exogenous NPM on endothelial cells. ( A ) The graph shows that the exogenous recombinant NPM (rNPM 200 ng/mL, w/rNPM) is able to foster migration of HUVEC. The 10% Fetal Bovine Serum (FBS) and the EBM TM -2 have been used as positive and negative stimulus, respectively (Pos Ctl, positive control; Neg Ctl, negative control). Below the graph are representative images of migrated cells. Magnification 4X. Data are the results of 5 independent experiments (technical duplicates). * p < 0.05, ** p < 0.01. ( B ) Proliferation assay (MTS assay) showing that the increased migration of HUVEC upon rNPM treatment is not associated to an enhancement of the proliferation up to 96 h. Data are the results of 4 independent experiments (technical duplicates). * p < 0.05. Complete medium has been used as the positive control (Pos Ctl). O.D., optical density, normalized vs. time 0. ( C ) FACS Analysis displaying scatter plots (top panel) with gating strategy to analyze only viable cells for ICAM-1 and VCAM-1 expression in the presence (w/rNPM) or in the absence of rNPM (w/o rNPM) treatment. Quantification plot (Bottom panel) showing that rNPM can significantly increase the percentage of positive HUVEC for ICAM-1 but not for VCAM-1. Data are the results of 4 independent experiments. ** p < 0.01.

Article Snippet: Cells were cultured in HUVEC complete growth medium (EGM TM -2 Endothelial Cell Growth Medium-2 BulletKit TM Lonza, Cat. N. CC-3162.

Techniques: Recombinant, Migration, Positive Control, Negative Control, Proliferation Assay, MTS Assay, Expressing

Journal: Stem Cells International

Article Title: Preconditioning by Hydrogen Peroxide Enhances Multiple Properties of Human Decidua Basalis Mesenchymal Stem/Multipotent Stromal Cells

doi: 10.1155/2018/6480793

Figure Lengend Snippet: The ability of DBMSCs to form tube networks and their inhibitory effects on HUVEC formation of tube networks. After 14 h, H 2 O 2 untreated DBMSCs (a) and DBMSC pretreated with 50 μ M H 2 O 2 (b) and 100 μ M H 2 O 2 (c); DBMSCs cultured in 50 μ M H 2 O 2 (d) or 100 μ M H 2 O 2 were unable to form tube networks. The addition of H 2 O 2 untreated DBMSCs (f) and DBMSC pretreated with 50 μ M H 2 O 2 (g) and 100 μ M H 2 O 2 (h) to HUVEC completely inhibited HUVEC formation of tube networks as compared to H 2 O 2 -untreated HUVEC (i). Experiments were carried out in triplicate DBMSCs (passage 3) prepared from five individual placentae.

Article Snippet: Cells were cultured in a complete HUVEC growth medium (catalogue number ATCC® PCS-100-041TM, ATCC, USA) at 37°C in a cell culture incubator.

Techniques: Cell Culture